EXPERIMENT #09
OBJECT:-
Retrieve a
sequence using UniprotKB.
THEORY:
What
is UniProt?
UniProt provides the scientific community with a comprehensive,
high-quality and freely accessible resource of protein sequence and functional
information. UniProt is a comprehensive, high-quality and freely
accessible database of protein sequence and functional information, many entries being derived from genome
sequencing projects. It contains a
large amount of information about the biological function of proteins derived
from the research literature.
Why
do we need UniProt?
As
the number of completely sequenced genomes continues to increase, huge efforts
are being made in the research community to understand as much as possible
about the proteins encoded by these genomes. This work is critical to many
areas of science including biology, medicine and biotechnology - and is
generating a wealth of data. UniProt
provides an up-to-date, comprehensive body of protein information. The resource
facilitates scientific discovery by collecting, interpreting and organising
this information, which saves researchers countless hours of work.
What
can I do with UniProt?
You can use UniProt for a wide range
of tasks, from finding out about your protein of interest and comparing its
protein sequence with other proteins, to mapping a list of identifiers
from an external database to UniProtKB
or vice versa.
UniProt is a comprehensive, high-quality and freely
accessible database of protein sequence and functional information, many entries being derived from genome
sequencing projects. It contains a
large amount of information about the biological function of proteins derived
from the research literature.
PROCEDURE:-
1.Www.uniprot.org/uniprot/P21543
was opened.
2.Name of
protein was written.
3.All the
information about protein was noted.
RESULTS:- 
DISCUSSION:-
UniProt is a comprehensive, high-quality and freely
accessible database of protein sequence and functional information, many entries being derived from genome
sequencing projects. It contains a
large amount of information about the biological function of proteins derived
from the research literature. It provides all the information related to that
protein(amylase),the name of organism (Bacillus
licheniformis), function of that protein. It showed the position of active
site of protein and also its graphical view. It gives information about the
position of signal peptide that was from 1-29 and functional chain that was at
30-512 position. Blue color showed alpha helix and red color showed Beta
strand. There were some repeated strands which meant that in evolution perhaps
there is duplication.
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