To observe N-glycosylation in eukaryotic sequence.

Experiment#11

Object:-

To observe N-glycosylation in eukaryotic sequence.

Theory:-

Most proteins do not perform their function without undergoing some form of post translational modification (PTM) . PTMs occur after the mRNA has been translated into peptide sequence and the polypeptide has begun to fold . The importance of PTMs in protein function makes their characterization of particular interest . Accurate prediction, using computational methods, of sites in a protein sequence where PTM occurs would facilitate protein annotation and would contribute to efforts in functional genomics Glycosylation , a common PTM, plays a role in protein folding, transport and half-life, as well as being involved in cell-cell interactions and antigenicity. Glycosylation is an enzymatic process, with the exception of glycation, and involves the addition of sugars to the protein to build up glycan chains. N-linked glycosylation consists of the addition of a pre-assembled glycan chain to Asn. This occurs co-translationally and influences protein folding. After its addition, the glycan chain undergoes a maturation process, which can produce a glycan of the high mannose, hybrid or complex types. The sequence motif Asn-Xxx-Ser/Thr , or in some rare cases Asn-Xxx-Cys, where Xxx is any amino acid except Pro, is required for N-glycosylation, although not sufficient on its own. This Web Service implements NetNGlyc 1.0b. It predicts N-Glycosylation sites in human proteins using artificial neural networks that examine the sequence context of Asn-Xaa-Ser/Thr sequons.The traditional server offers more detailed output (graphics), extended functionality and comprehensive documentation. It is suitable for close investigation of few proteins while this service is recommended for high throughput projects.

Procedure:-

1.NetNGly1.0 server was opened.

2. Fasta format was pasted in input box.

3.Result was noted.

DISCUSSION:-

In this experiment N-Glycosylation was observed. This Web Service implements NetNGlyc 1.0b. It predicts N-Glycosylation
sites in human proteins using artificial neural networks that examine
the sequence context of Asn-Xaa-Ser/Thr sequons.

The traditional server offers more detailed output (graphics), extended
functionality and comprehensive documentation. Glycosylation mainly depends on nature of amino acids and environment around specific aminoacids .N glycosylation occur where basic aminoacids like Asparigin is present.We observed that N glycosylation occurred at many positions of given sequence.

Discussion:-

In this experiment N-Glycosylation was observed. N-linked glycosylation consists of the addition of a pre-assembled glycan chain to Asn. This occurs co-translationally and influences protein folding. After its addition, the glycan chain undergoes a maturation process, which can produce a glycan of the high mannose, hybrid or complex types. The sequence motif Asn-Xxx-Ser/Thr , or in some rare cases Asn-Xxx-Cys, where Xxx is any amino acid except Pro, is required for N-glycosylation, although not sufficient on its own. The traditional server offers more detailed output (graphics), extendedfunctionality and comprehensive documentation.We observed that N glycosylation occurred at many positions of given sequence.